Thermo Fisher Scientific DDX47 Polyclonal Antibody
Rabbit polyclonal antibody against human DDX47 protein. Validated for WB, IHC-P, and IP applications. High specificity and affinity-purified. Supplied as liquid in PBS with BSA and glycerol. Suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | — |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | — |
| Immunoprecipitation (IP) | Assay-dependent | — |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 243–455 of human DDX47 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549185 |
Product Specific Information
- Recommended positive control: IMR32
- Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (86%), Chicken (89%), Chimpanzee (100%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The DDX47 gene encodes a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are putative RNA helicases involved in various cellular processes requiring alteration of RNA secondary structure, including translation initiation, splicing, and ribosome assembly. Some family members play roles in embryogenesis, spermatogenesis, and cellular growth and division. DDX47 can shuttle between the nucleus and cytoplasm and exhibits RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been identified.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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