Thermo Fisher Scientific Mouse IL-1 beta (ELISA Standard) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species

Mouse

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

Mouse IL-1 beta, amino acids Val118-Ser269 (Accession # P10749)

Molecular weight

17 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Description: mouse IL-1 beta, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1 beta is mostly cell associated with 23% amino acid homology with IL-1 alpha. The immune regulatory role of IL-1 beta is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported: Mouse IL-1 beta RSG ELISA Lyophilized Standard has been reported for use in the Mouse IL-1 beta ELISA RSG (Product # 88-7013).

Applications Tested: The intended use of the Mouse IL-1 beta RSG ELISA Lyophilized Standard is as a standard for Mouse IL-1 beta ELISA (Product # 88-7013-88). Typically the standard is run in a series of 7 doubling dilutions from the top standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard.

Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate.

Source: E. coli expressed amino acids Val 118-Ser 269 of mouse IL-1 beta accession # P10749.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.