
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합된 염소 유래 항-토끼 IgG(H+L) 2차 항체로 Western blot, IHC, ELISA, IP에 적합합니다. 교차흡착 처리로 비특이적 결합 최소화. 동결건조 형태로 제공되며 -20°C 보관. 연구용으로 높은 민감도와 신호 증폭 효과 제공.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:200,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2536530 |
| Target | IgG |
| Cross Adsorption | Against human IgG, human serum, mouse IgG, mouse serum, and bovine serum |
| Antibody Form | Whole Antibody |
Product Specific Information
- Formulation update: As of March 31, 2024, formulation changed from PBS with 0.02% thimerosal to PBS without thimerosal. Lots >2855945 do not contain thimerosal.
- Storage: Store at -20°C until opened.
- Reconstitution: Add 1 mL PBS (pH 7.2) to yield a 1 mg/mL stock.
- Stability:
- Without stabilizer: Stable ~3 months at 4°C.
- With 50% glycerol (v/v): Stable up to 1 year at -20°C.
- With preservative (e.g., 0.02% thimerosal): Stable up to 1 year at 4°C.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and specifically recognize rabbit immunoglobulins. They are widely used for detection, sorting, or purification of rabbit targets.
Secondary antibodies enhance detection versatility (HRP, AP, fluorescence) and improve sensitivity through signal amplification.
Typically, they are generated by immunizing host animals with rabbit immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.
Available Conjugate Formats
This antibody is available in multiple conjugated forms for diverse detection applications:
- Alexa Fluor series (350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790)
- DyLight series (350, 405, 488, 550, 594, 633, 650, 680, 755, 800)
- FITC, PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647
- Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X, TRITC
- Biotin, Biotin-XX
- Cyanine3, Cyanine5
- Pacific Blue, Pacific Orange
- AP, APC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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