Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), eFluor 450, eBioscience
Thermo Fisher Scientific의 CD1d Monoclonal Antibody (1B1), eFluor 450은 마우스 CD1d 단백질 검출용 항체로, 유세포 분석(Flow Cytometry)에 최적화되어 있습니다. Violet Laser(405 nm)에서 여기되어 445 nm에서 방출되며, 0.25 µg/test로 사용 가능합니다. 연구용으로만 사용됩니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), eFluor 450, eBioscience
Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 정보 없음
- View 2 publications
Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
- View 5 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B1 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574003 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- Brilliant Violet 480
- Brilliant Violet 711
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with beta2m and functionally resembles MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes and recognizes beta2m-associated CD1d.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.25 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility.
Laser: Violet Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD1d is a heavy chain associated with Beta-2 microglobulin on cortical thymocytes. It can also be expressed independently on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. These proteins present lipid and glycolipid antigens to T cells. The CD1d gene, located on chromosome 1, encodes a transmembrane glycoprotein that localizes to late endosomes and lysosomes via a tyrosine-based motif.
Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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