Thermo Fisher Scientific Rabbit anti-Goat IgG Recombinant Secondary Antibody, Alexa Fluor Plus 488

Thermo Fisher Scientific Rabbit anti-Goat IgG Recombinant Secondary Antibody, Alexa Fluor Plus 488

Applications and Tested Dilution

Product Specifications

Product Specific Information

Recombinant antibodies are produced using genes that code for the desired antibodies, cloned into an expression vector and expressed in vitro.
Advantages include:

• Better specificity
• Superior lot-to-lot consistency

This recombinant antibody has engineered point mutations that eliminate Fc receptor binding, reducing background and improving signal-to-noise ratio.

Alexa Fluor Plus secondary antibodies deliver brighter fluorescence compared to conventional Alexa Fluor conjugates. They utilize proprietary dye chemistry for enhanced imaging performance, ideal for detecting low-abundance targets.

Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to minimize nonspecific staining. Optimize dilution empirically based on application.

Target Information

Anti-Goat secondary antibodies are affinity-purified with high specificity for goat immunoglobulins.
Applications include:

• Detection
• Sorting
• Purification

These antibodies enable flexible detection systems (HRP, AP, fluorescence) and amplify signals by binding multiple secondary antibodies to one primary antibody. Typically generated by immunizing host animals with pooled goat immunoglobulins, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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