Thermo Fisher Scientific Rabbit anti-Mouse IgG Recombinant Secondary Antibody, Alexa Fluor Plus 647

Applications

Tested Dilution

Western Blot (WB)

1-2 µg/mL

Immunocytochemistry (ICC/IF)

0.5-4 µg/mL

Flow Cytometry (Flow)

1-4 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

35H35L37

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

View additional formats

• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG/conjugates/Alexa Fluor Plus 488/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

658/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.5

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2930913

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity and superior lot-to-lot consistency.

Minimal cross-reactivity with Human, Goat, Donkey, Guinea Pig, Horse and Bovine IgG observed.

This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.

Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.