Thermo Fisher Scientific CDC27 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CDC27 protein. Validated for Western blot and immunoprecipitation. Recognizes homologous proteins in mouse, rat, and bovine. Liquid form, stored at 4°C, shelf life 1 year.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 774 and 824 of human cell division cycle 27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Predicted to react with Mouse, Rat, and Bovine (based on 100% sequence identity)
Target Information
The CDC27 gene encodes a protein that is a component of the anaphase-promoting complex (APC), composed of eight highly conserved subunits in eukaryotic cells. APC catalyzes the formation of cyclin B–ubiquitin conjugates, essential for ubiquitin-mediated proteolysis of B-type cyclins.
CDC27 and other APC components contain TPR (tetratricopeptide repeat) domains, facilitating protein–protein interactions. This protein interacts with mitotic checkpoint regulators such as Mad2, p55CDC, and BUBR1, contributing to mitosis timing control.
Alternative splicing results in multiple isoforms of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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