Thermo Fisher Scientific TBX3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

1:1,000

-

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of human T-box 3, which differs from mouse and rat by one conservative and one non-conservative amino acid

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533526

Product Specific Information

42-4800 has been successfully used in immunohistochemistry analysis of Tbx3 in mouse E16.5 frozen lung sections.

Target Information

T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.