Thermo Fisher Scientific Goat anti-Human IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor Plus 488

Applications

Tested Dilution

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:500 - 1:1,000

Flow Cytometry (Flow)

1:1,000

Immunoradiometric Assay (IRMA)

1:1,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Secondary Antibody

Immunogen

Purified Human IgG

Conjugate

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Human IgG (Heavy Chain)/conjugates/Alexa Fluor 488/host/Goat/)
• [HRP](/antibody/secondary/product-name/Human IgG (Heavy Chain)/conjugates/HRP/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

493/518 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.5

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_2937235

Target

IgG (Heavy chain)

Antibody Form

Whole Antibody

Product Specific Information

This Goat-anti-Human IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody specifically detects human IgG Heavy chain with no cross-reactivity to light chain and other species immunoglobulins except moderate cross-reactivity towards Monkey IgG.

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

Superclonal Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.

The sensitivity and specificity of each lot is confirmed using ELISA.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.