Thermo Fisher Scientific Desmoglein-3 (Squamous Cell Marker) Monoclonal Antibody (5G11)
Desmoglein-3 단백질을 인식하는 단클론 항체로, 편평상피세포 마커로 사용됩니다. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 인간 시료에 반응합니다. 단백질 A/G로 정제된 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G11 |
| Immunogen | Recombinant human DSG3 extracellular protein fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominantly expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus.
Desmoglein 3 is the autoantigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, resulting in blister formation on the skin and oral mucosa, and histologically as suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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