Thermo Fisher Scientific CD45 Recombinant Rabbit Monoclonal Antibody (4R0B0)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4R0B0

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 194-390 of human CD45 (NP_0028293) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD45,uniProtId:P08575-3,ncbiNodeId:9606,antigenRange:194-390,antigenLength:1306,antigenImageFileName:MA5-35206_CD45_P08575-3_Rabbit.svg,antigenImageFileNamePDP:MA5-35206_CD45_P08575-3_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2849110

Product Specific Information

Immunogen sequence: SDAYLNASET TTLSPSGSAV ISTTTIATTP SKPTCDEKYA NITVDYLYNK ETKLFTAKLN VNENVECGNN TCTNNEVHNL TECKNASVSI SHNSCTAPDK TLILDVPPGV EKFQLHDCTQ VEKADTTICL KWKNIETFTC DTQNITYRFQ CGNMIFDNKE IKLENLEPEH EYKCDSEILY NNHKFTNASK IIKTDFG

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.