Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI4E1), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Ubiquitin is a highly conserved protein that plays a key role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.
The UBA52 gene encodes a fusion protein with ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, post-translational processing generates free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S ribosomal subunit, belonging to the S27AE family, containing C4-type zinc finger domains and localized in the cytoplasm. Pseudogenes derived from this gene exist in the genome.
Similar fusion mechanisms occur for ribosomal proteins L40 and S30, which are synthesized with ubiquitin or ubiquitin-like proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.