Thermo Fisher Scientific c-Met Monoclonal Antibody (eBioclone 7), FITC, eBioscience

Thermo Fisher Scientific c-Met Monoclonal Antibody (eBioclone 7), FITC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1 µg/test
  View 4 publications

Product Specifications

Additional Formats

• Unconjugated
• PE
• Request custom conjugation

Product Specific Information

The eBioclone 7 monoclonal antibody was generated against a mouse c-Met-Ig fusion protein and reacts with mouse c-Met-transfected cells.
Mouse c-Met (HGFR) is a 170 kDa receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver, and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation and activation of downstream effectors including ERK-1, ERK-2, and PLCγ.
Activation of the c-Met signal transduction pathway leads to cellular responses such as motility, scattering, proliferation, survival, and angiogenesis.
Mutations in human c-Met are implicated in several malignancies.

Applications Reported:
This eBioclone 7 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse c-Met-transfected 293T cells. Recommended usage ≤ 1 µg per test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test.
Careful titration is recommended for optimal assay performance.

Optical Properties:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation is associated with various human cancers. Upon activation, MET interacts with PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3, triggering signaling cascades including RAS-ERK, PI3K-AKT, and PLCγ-PKC.
MET plays roles in embryonic development (gastrulation, muscle and neuron development, angiogenesis, kidney formation) and adult processes (wound healing, organ regeneration, tissue remodeling).
Aberrant MET activity is linked to gastric, renal, and breast cancers, making it a key target for cancer therapeutics and diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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