Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 605 / 650 / 711
• eFluor 450 / 506
• FITC
• NovaFluor Blue / Red / Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except circulating erythrocytes and platelets. It plays a crucial role in lymphocyte activation through its tyrosine phosphatase activity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry of normal human peripheral blood cells
• Recommended Usage: 5 µL (0.25 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers with a violet (405 nm) laser. Ensure instrument compatibility prior to use.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant UV™, Super Bright™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It is absent in non-hematopoietic cells and tissues. CD45 is essential for T- and B-cell receptor signaling, cytokine receptor regulation, and plays roles in apoptosis and integrin-mediated signal transduction. Multiple isoforms exist due to alternative splicing, differing in extracellular domains.

For Research Use Only. Not for use in diagnostic procedures.

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