Thermo Fisher Scientific Rubicon Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Rubicon (RUN domain Beclin-1 interacting and cysteine-rich containing protein) interacts with Beclin-1 and ATG14L, regulating autophagy at different stages.

• Knockdown of Rubicon enhances autophagy, while ATG14L knockdown causes defects in autophagosome formation.
• Rubicon is part of a Beclin-1–PIK3C3-containing autophagy complex and acts as a positive regulator of the NADPH oxidase complex.
• Upon microbial infection or TLR2 activation, Rubicon interacts with CYBA subunit, inducing reactive oxygen species and inflammatory cytokine production.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.