Thermo Fisher Scientific LIMK1/LIMK2 Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 Rabbit Polyclonal Antibody로 LIMK1/LIMK2 단백질 검출에 사용. Western Blot, IHC, ICC/IF 등 다양한 응용에 적합. Affinity chromatography로 정제된 고순도 항체. 세포 이동성과 형태 형성 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LIMK1 (Accession P53667), corresponding to amino acid residues A482–V532 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818467 |
Product Specific Information
Antibody detects endogenous levels of total LIMK1/2.
Target Information
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 regulates stabilization of F-actin structures and cofilin activity, playing a role in signaling pathways involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, acting downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest different functions during development. A truncated form of LIMK-2 has been identified in adult testis, thought to arise from an alternative initiation exon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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