Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Functional Grade, eBioscience
Human CD86 (B7-2) 단백질을 인식하는 IT2.2 클론 단일클론 항체로, T 세포 활성화 및 면역반응 연구에 적합. Flow cytometry 및 기능적 차단 실험에 사용 가능. 고순도(>90%) 및 저엔도톡신 수준으로 제조된 Functional Grade 제품.
- 카탈로그번호
- 16-0869-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Functional Grade, eBioscience
Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Functional Grade, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 22 publications |
| Neutralization (Neu) | Assay-Dependent | View 2 publications |
| Functional Assay | Assay-Dependent | - |
| Inhibition Assays (IA) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Functional Grade |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IT2.2 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865807 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 647, 700
- APC, Biotin
- Brilliant UV 737, Brilliant Violet 480, 711, 786
- NovaFluor Blue 660-120S, Red 700, Red 725, Red 755, Yellow 610, Yellow 690, Yellow 700, Yellow 755
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 645
- Request custom conjugation available
Product Specific Information
The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor belonging to the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated upon stimulation through BCR, CD40, and cytokine receptors.
CD86 interacts with CD28 and CTLA-4 on T cells, playing a crucial role in T-B cell crosstalk, T cell costimulation, and antibody production. Its rapid upregulation supports its role in the primary immune response.
Applications Reported: Flow cytometry and functional blocking studies.
Applications Tested: Flow cytometric analysis of human peripheral blood cells. Recommended ≤0.25 µg/test (100 µL volume). Optimize antibody titration for each assay.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
CD86 is one of two ligands (with CD80) for CTLA-4 and CD28, functioning as a costimulatory molecule during antigen presentation. Insufficient CD86/CD80 co-stimulation can induce tolerance.
CD86 is a type I membrane protein and member of the immunoglobulin superfamily, expressed by antigen-presenting cells.
Binding with CD28 activates T cells, while interaction with CTLA-4 inhibits activation.
Alternative splicing yields multiple isoforms.
Associated diseases include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.