Thermo Fisher Scientific PKC Pan Polyclonal Antibody
PKC 계열 단백질을 검출하기 위한 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ICC/IF, ELISA에 적합하며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 572–672 of human PRKCA (NP_0027282) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.27 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914165 |
Product Specific Information
Positive test controls include:
- HeLa
- Jurkat
- 293T
- Mouse brain
- Rat brain
Immunogen sequence:
MTKHPAKRLG CGPEGERDVR EHAFFRRIDW EKLENREIQP PFKPKVCGKG AENFDKFFTR GQPVLTPPDQ LVIANIDQSD FEGFSYVNPQ FVHPILQSAV
Target Information
Members of the protein kinase C (PKC) family play key regulatory roles in various cellular functions, including:
- Cell growth and differentiation
- Gene expression
- Hormone secretion
- Membrane function
PKCs are serine/threonine kinases activated by calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters activate PKC.
PKC isoforms are classified as:
- Conventional (cPKC): α, βI, βII, γ
- Novel (nPKC): δ, ε, ζ, η, θ
Each isoform shows distinct tissue expression and cofactor dependency; for example, nPKC δ and ε kinase activities are independent of Ca²⁺.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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