Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor Red 685, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.6 µg)/test

Species Reactivity

• Human

Host / Isotype

• Mouse / IgG1, kappa

Class

• Monoclonal

Type

• Antibody

Clone

• CEW33D

Conjugate

• NovaFluor™ Red 685

Additional Formats Available

• APC
• Biotin
• Brilliant UV 615
• eFluor 660
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• Custom conjugation available

Spectral Properties

Physical Properties

Product Specific Information

The CEW33D monoclonal antibody reacts with human CD183 (CXCR3), a G protein-coupled chemokine receptor interacting with CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). CD183 is associated with type 1 immunity, expressed in activated T cells, Th1 cells, CD8 effector cells, NK and NKT cells, and some B cells and plasmacytoid dendritic cells. Its ligand interactions mediate tissue infiltration in inflammatory and autoimmune responses.

Applications Reported:

• Suitable for flow cytometric analysis.

Applications Tested:

• Pre-titrated and tested with normal human peripheral blood cells.
• Use 5 µL (0.6 µg) per test in a final volume of 100 µL.
• Recommended cell range: 10⁵–10⁸ cells/test.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
• Add 5 µL of CellBlox Plus Buffer per sample or per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescence performance with minimal spillover.

Target Information

The CD183 (CXCR3) gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These CXC chemokines induce leukocyte trafficking, integrin activation, cytoskeletal changes, and chemotaxis. The receptor is expressed in effector/memory T cells and inflamed tissues. It is a potential therapeutic target for inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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