
Thermo Fisher Scientific NeutrAvidin Protein, DyLight 550
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Applications
Tested Dilution
Publications
Immunocytochemistry (ICC/IF)
Assay-dependent
Product Specifications
Type
Protein
Conjugate
DyLight™ 550 DyLight™ 550 DyLight™ 550
Excitation/Emission Max
556/570 nm
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
Buffered saline solution with 1% BSA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient
Target Information
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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