Thermo Scientific™

SuperSignal™ Enhanced Molecular Weight Protein Ladder

Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with자세히 알아보기

Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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The ladder`s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).

Two versions of the MW marker are offered. This formulation should be used in applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody. The SuperSignal Molecular Weight Protein ladder formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies.

사양

검출 방법User defined detection system

Number of Markers8

제품 유형Protein Ladder

로드 준비Yes

크기 범위20 to 150 kDa

염색 유형Unstained

젤 호환성Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

분자량150, 100, 80, 60, 50, 40, 30, 20 kDa

제품라인SuperSignal™

수량250 μL

배송 조건Wet Ice

시스템 유형Western Blotting, SDS-PAGE

Unit SizeEach