Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체. Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 1 mg/mL 액상 제품. 대부분의 조직에서 발현되는 GAPDH 검출에 유용.
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Thermo Fisher Scientific GAPDH Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 and 225 of human Glyceraldehyde-3-Phosphate Dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted reactivity based on sequence identity: Rat, D. melanogaster, Zebrafish, X. laevis, Chicken, Sheep, Bovine, Rabbit, Guinea pig, Pig, Golden hamster, Cat
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It forms a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphophate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, repair, RNA export, membrane fusion, and microtubule bundling. It plays roles in apoptosis, neurodegenerative diseases, and cancer. GAPDH interacts with proteins such as amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease).
Due to its ubiquitous expression, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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