Thermo Fisher Scientific HLA-DOA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.04–0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200–1:500

Product Specifications

Product Specific Information

• Immunogen sequence:
  GDFARFDPQG GLAGIAAIKA HLDILVERSN RSRAINVPPR VTVLPKSRVE
• Highest antigen sequence identity to orthologs:
  • Mouse: 76%
  • Rat: 70%

Target Information

HLA-DOA antibody reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively.
DO forms tight complexes with DM in the endoplasmic reticulum and is sorted to lysosomal vesicles during antigen processing and presentation. It is selectively expressed on antigen-presenting cells such as B cells, dendritic cells, and thymic epithelial cells.
DO enhances peptide loading efficiency and stabilizes DM at low pH, preserving its chaperone activity. DO-DM complexes are more efficient than DM alone in protecting empty DR molecules, functioning as a co-chaperone of DM.

HLA and MHC antibodies are crucial in Immunopeptidomics, aiding in the identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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