Thermo Fisher Scientific ErbB3 Monoclonal Antibody (2F9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F9

Immunogen

Purified recombinant extracellular fragment of human ERBB3 (aa22-369) fused with hIgGFc tag expressed in HEK293 cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Her-3 (ErbB3),uniProtId:P21860-1,ncbiNodeId:9606,antigenRange:22-369,antigenLength:1342,antigenImageFileName:MA5-15536_Her-3_ErbB3_P21860-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15536_Her-3_ErbB3_P21860-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10985508

Product Specific Information

MA5-15536 targets ERBB3 in indirect ELISA, IF and WB applications and shows reactivity with Human samples.

The MA5-15536 immunogen is purified recombinant extracellular fragment of human ERBB3 (aa22-369) fused with hIgGFc tag expressed in HEK293 cells.

MA5-15536 detects ERBB3 which has a predicted molecular weight of approximately 148kDa.

Target Information

ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.