Thermo Fisher Scientific ErbB3 Monoclonal Antibody (2F9)
Human ERBB3 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, ICC/IF, ELISA에 적합. 비결합형 액상 형태이며, 단기 4°C 보관, 장기 -20°C 보관 권장. 세포 신호전달 및 종양 연구용으로 최적화됨.
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Thermo Fisher Scientific ErbB3 Monoclonal Antibody (2F9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F9 |
| Immunogen | Purified recombinant extracellular fragment of human ERBB3 (aa22-369) fused with hIgGFc tag expressed in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10985508 |
Product Specific Information
MA5-15536 targets ERBB3 in indirect ELISA, IF, and WB applications and shows reactivity with Human samples.
The immunogen is a purified recombinant extracellular fragment of human ERBB3 (aa22–369) fused with hIgGFc tag expressed in HEK293 cells.
MA5-15536 detects ERBB3 with a predicted molecular weight of approximately 148 kDa.
Target Information
ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors that binds to ligands in the heregulin family.
ErbB3 is over-expressed in various tumors including prostate, bladder, breast, stomach, pancreas, and colon.
Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
The ErbB3 gene is located on chromosome 12q13 and encodes a 160/180 kDa transmembrane glycoprotein receptor tyrosine kinase.
It forms heterodimers with other EGF receptor family members, activating pathways for cell proliferation or differentiation.
Alternative splicing generates different isoforms, including a secreted form that modulates membrane-bound ErbB3 activity.
Additional splice variants have been reported but are not fully characterized.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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