Thermo Fisher Scientific APPL1 Monoclonal Antibody (OTI4B11), TrueMAB
APPL1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P)에서 검증됨. 인간 시료 반응성, 동결건조 형태, 보존제 무첨가. 세포 신호전달 연구 및 단백질 상호작용 분석에 적합.
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Applications
- Western Blot (WB): 1:2,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B11 |
| Immunogen | Full length human recombinant protein of human APPL1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The APPL1 protein is involved in regulating cell proliferation and mediating crosstalk between adiponectin and insulin signaling pathways.
It interacts with multiple proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and components of the NuRD/MeCP1 complex.
APPL1 is associated with endosomal membranes but can translocate to the nucleus upon EGF stimulation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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