Thermo Fisher Scientific Pumilio 1 Polyclonal Antibody
Pumilio 1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 고순도 Protein A 정제, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Pumilio 1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Pumilio 1 (amino acids 201–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It belongs to the PUF family of evolutionarily conserved RNA-binding proteins. PUM1 binds to the RNA consensus sequence known as the Pumilio Response Element (PRE, 5'-UGUANAUA-3'), related to the Nanos Response Element (NRE). It mediates post-transcriptional repression through recruitment of the CCR4-POP2-NOT deadenylase complex, leading to translational inhibition and mRNA degradation. Mutations in this gene are associated with spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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