Thermo Fisher Scientific Goat anti-Rabbit IgG (Heavy chain) Superclonal Secondary Antibody, Alexa Fluor 488

Applications

Tested Dilution

Western Blot (WB)

1:2,000-1:5,000

Immunohistochemistry (Frozen) (IHC (F))

1:2,000

Immunocytochemistry (ICC/IF)

1:250-1:2,000

Flow Cytometry (Flow)

1-5 µg/mL

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Secondary Antibody

Immunogen

Recombinant full-length protein

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/Biotin/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (Heavy chain)/conjugates/HRP/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Gravity column chromatography

Storage buffer

PBS

Contains

5mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2536097

Target

IgG (Heavy chain)

Antibody Form

Whole Antibody

Product Specific Information

The sensitivity and specificity of each lot is confirmed using ELISA.

Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig and donkey IgG is observed.

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.