Thermo Fisher Scientific Phospho-ATF2 (Thr53, Thr71) Polyclonal Antibody
Western blot과 IHC에 적합한 다클론 항체로, 인간, 마우스, 랫트 시료에서 ATF2 인산화(Thr53, Thr71) 검출에 사용됩니다. Protein A로 정제된 액상 항체이며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence including phosphorylation sites of threonine 71 or 53 (T-P-T(p)-P-T) derived from human ATF2 and conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | Dulbecco’s PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with various viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulates ATF-2 transcriptional activity.
Activation occurs through phosphorylation of Thr69 and Thr71 by SAPK and p38 MAPK in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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