Thermo Fisher Scientific Calpain 3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length Human Calpain 3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpain 3,uniProtId:P20807-1,ncbiNodeId:9606,antigenRange:1-821,antigenLength:821,antigenImageFileName:PA5-76774_Calpain_3_P20807-1_Rabbit.svg,antigenImageFileNamePDP:PA5-76774_Calpain_3_P20807-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2720501

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, plays a role in cytoskeletal reorganization and muscle protein degradation. Calpain is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated calpain 1, calpain 2 and calpain 3. Calpain 3 (calpain p94) is a muscle-preferred calcium activated neutral protease that localizes to the nucleus. Mutations involving the calpain 3 gene are associated with limb-girdle muscle dystrophy type 2A, a form of autosomal recessive and progressive neuromuscular diso4. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.