Thermo Fisher Scientific Calpain 3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

Calpain is an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, playing a role in cytoskeletal reorganization and muscle protein degradation. It is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits: calpain 1, calpain 2, and calpain 3.
Calpain 3 (calpain p94) is a muscle-preferred calcium-activated neutral protease that localizes to the nucleus. Mutations in the calpain 3 gene are associated with limb-girdle muscular dystrophy type 2A, an autosomal recessive and progressive neuromuscular disorder.
Calpastatin regulates calpain by inhibiting both its proteolytic activity and membrane binding. Calpastatin exists in tissue and erythrocyte types, resulting from alternative splicing and proteolytic processing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.