Thermo Fisher Scientific ACAT2 Monoclonal Antibody (OTI3A11), TrueMAB
ACAT2 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 인간 시료에 반응하며, 동결건조 형태로 제공. 고순도 친화 크로마토그래피 정제 및 트레할로스 안정화 PBS 버퍼 포함.
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Thermo Fisher Scientific ACAT2 Monoclonal Antibody (OTI3A11), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A11 |
| Immunogen | Full-length human recombinant ACAT2 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
This gene encodes a member of the frizzled gene family, which includes 7-transmembrane domain proteins acting as Wnt signaling receptors. The encoded protein has a signal peptide, a cysteine-rich domain, and seven transmembrane domains but lacks a C-terminal PDZ domain-binding motif. It functions as a negative regulator of the canonical Wnt/β-catenin signaling pathway, inhibiting oncogenic transformation, cell proliferation, and apoptosis suppression. Alternative splicing produces multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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