Thermo Fisher Scientific BTRC Polyclonal Antibody
Thermo Fisher Scientific의 BTRC Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 항체입니다. Western blot, IHC, ICC, ELISA에 적합하며, 고순도의 액상 형태로 제공됩니다. Affinity Chromatography로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–100 of human β-TrCP/BTRC (NP_3786631) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2854870 |
Product Specific Information
Immunogen sequence:
MDPAEAVLQE KALKFMCSMP RSLWLGCSSL ADSMPSLRCL YNPGTGALTA FQNSSEREDC NNGEPPRKII PEKNSLRQTY NSCARLCLNQ ETVCLASTAM
Target Information
This gene encodes a member of the F-box protein family characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins are subunits of the ubiquitin protein ligase complex SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
F-box proteins are divided into three classes:
- Fbws containing WD-40 domains
- Fbls containing leucine-rich repeats
- Fbxs containing other interaction modules or no recognizable motifs
The encoded protein belongs to the Fbws class, containing multiple WD-40 repeats. It is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2, and Drosophila Slimb proteins. It interacts with HIV-1 Vpu, connecting CD4 to the proteolytic machinery, and associates with phosphorylated IkappaBalpha and beta-catenin destruction motifs. This protein likely functions in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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