Thermo Fisher Scientific Human GUSB (Beta Glucuronidase) Endogenous Control (VIC/TAMRA probe, primer limited)

Applied Biosystems™ Human GUSB (Beta Glucuronidase) Endogenous Control (VIC™/TAMRA™ Probe, Primer Limited)

The Applied Biosystems™ Human GUSB (beta glucuronidase) Endogenous Control (VIC™/TAMRA™ Probe, Primer Limited) is intended as an endogenous control for relative gene expression quantification in cDNA samples when used with other TaqMan™ gene expression assays.
Probe is labeled with VIC™ dye and TAMRA™ dye, with primers limited for multiplex or singleplex PCR reactions.
Compatible with Inventoried and Made-to-Order TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate months of assay design, formulation, and testing. These pre-designed probe and primer sets enable normalization of RNA or DNA amounts in reactions for accurate gene expression quantification.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls simplify the selection of appropriate controls for human, mouse, rat, and other eukaryotic genes, reducing trial-and-error in control validation.

Simple to Use

All components are QC tested and formulated into a single 20X mix. Compatible with universal conditions for two-step RT-PCR.
Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and cDNA samples for sensitive, reproducible, and quantitative gene expression on Applied Biosystems instruments including 7900HT, 7300, 7500, 7000, and 7700 systems.

Flexible Offering

Each endogenous control uses 5′ nuclease chemistry for flexibility. Available configurations include:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled PCR primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled PCR primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM) with two unlabeled PCR primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting cDNA input variations. Ensure uniform expression across all samples.
For multiplexing, the endogenous control gene should be more abundantly expressed than the target gene.

Multiplex vs. Singleplex PCR

TaqMan™ Endogenous Controls with VIC™ reporter dye are primer limited for multiplexing with target assays.
Controls with FAM™ reporter dye are not primer limited and not intended for multiplexing.

For Research Use Only. Not for use in diagnostic procedures.

제품 사양

제품 이미지