Thermo Fisher Scientific Thioredoxin 1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Immunogen sequence:
VKQIESKTAF QEALDAAGDK LVVVDFSATW CGPCKMIKPF FHSLSEKYSN VIFLEVDVDD CQDVASECEV KCMPTFQFFK KGQKVGEFSG ANKEKLEATI NELV

Target Information

Thioredoxins (Trx) are small, multifunctional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds.

Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx acts as a hydrogen donor for ribonucleotide reductase, an essential enzyme for DNA synthesis, and functions as a potent general protein disulfide reductase involved in growth and redox regulation.

Specific protein disulfide targets for reduction by Trx include:

• Protein disulfide-isomerase (PDI)
• Transcription factors such as p53, NF-κB, and AP-1 (T1-151)

Trx is also capable of removing H₂O₂, particularly when coupled with methionine sulfoxide reductase or several isoforms of peroxiredoxins.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.