Thermo Fisher Scientific MDM4 Monoclonal Antibody (OTI4G5), TrueMAB
MDM4 단백질을 인식하는 Mouse 모노클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human 시료에 반응하며, Affinity chromatography로 정제된 액상 형태. p53 조절 관련 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G5 |
| Immunogen | Full length human recombinant protein of human MDM4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The MDM2 protein is the primary regulator of p53 protein stability. MDMX (also known as MDM4) is an MDM2-related protein that inhibits MDM2-mediated degradation of p53 via distinct associations with MDM2. The gene encoding MDMX is often amplified in malignant gliomas. ARF interacts with MDMX to sequester it within the nucleolus, leading to increased p53 transactivation. Expression of MDMX can counteract MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation. Like MDM2, MDMX also binds and stabilizes p73, increasing its half-life.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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