Thermo Fisher Scientific Mouse IL-4, Animal-Free Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

ELISA (ELISA)

-

View 3 publications 3 publications

Functional Assay (Functional)

Assay-dependent

-

In vitro Assay (IV)

-

View 115 publications 115 publications

Miscellaneous PubMed (Misc)

-

View 7 publications 7 publications

Product Specifications

Species

Mouse

Published species

Fish, Human, Mouse

Expression System

E. coli

Amino acid sequence

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Molecular weight

13.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5 units/mg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine IL-4 is a 13.5 kDa globular protein containing 121 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.