Thermo Fisher Scientific Smoothelin Polyclonal Antibody
Smoothelin 단백질을 표적으로 하는 Thermo Fisher Scientific의 rabbit polyclonal antibody. Western blot에 사용 가능하며, 인간·마우스·랫트 반응성. 항원 친화 크로마토그래피로 정제, 고순도 (>95%). 평활근 세포 분화 연구에 적합.
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Thermo Fisher Scientific Smoothelin Polyclonal Antibody
Applications
- Western Blot (WB): 1:500–1:2,000
Species Reactivity
- Human
- Mouse
- Rat
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length Human Smoothelin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720738 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle, do not contain smoothelin.
Confocal microscopy and transfection studies show a filamentous organization of smoothelin distinct from desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue-specific isoforms have been identified:
- 59 kDa isoform (visceral SMC specific)
- 100 kDa isoform (vascular SMC specific)
Human smoothelin is encoded by a single-copy gene located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-77011_Smoothelin_P53814-1_Rabbit_PDP.jpeg)
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