Thermo Fisher Scientific Smoothelin Polyclonal Antibody

Thermo Fisher Scientific Smoothelin Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,000

Species Reactivity

• Human
• Mouse
• Rat

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle, do not contain smoothelin.

Confocal microscopy and transfection studies show a filamentous organization of smoothelin distinct from desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue-specific isoforms have been identified:

• 59 kDa isoform (visceral SMC specific)
• 100 kDa isoform (vascular SMC specific)

Human smoothelin is encoded by a single-copy gene located on chromosome 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지: PA5-77011_Smoothelin_P53814-1_Rabbit_PDP.jpeg)