Thermo Fisher Scientific V5 Tag Monoclonal Antibody (A10-D0-A1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

ELISA (ELISA)

1:20,000-1:50,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A10-D0-A1

Immunogen

Peptide GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931093

Product Specific Information

Application notes: Indirect ELISA

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.