Thermo Fisher Scientific Vinculin Monoclonal Antibody (SP117)
Rabbit monoclonal antibody (clone SP117) targeting human Vinculin. Validated for IHC (paraffin) with 1:100 dilution. Recommended for research use in cell adhesion and cytoskeletal studies. Supplied in PBS with 1% BSA, stored at 4°C or -20°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP117 |
| Immunogen | Synthetic peptide derived from C-terminus of human vinculin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537943 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is heart or small intestine tissue.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane.
In adhesion plaque assembly, the beta subunit of integrin binds to talin, which binds to vinculin that interacts with alpha-actinin and possibly with itself.
Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
Mutations in the VCL gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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