Thermo Fisher Scientific KRT19 Monoclonal Antibody (OTI5G7), TrueMAB
인간 KRT19 단백질(aa 240-390)을 항원으로 한 Mouse IgG1 단클론 항체. Western blot 및 IHC(P)에서 검증됨. 동결건조 형태로 제공되며, PBS와 trehalose buffer에 보존. 세포 분화 및 상피성 종양 연구에 유용.
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Thermo Fisher Scientific KRT19 Monoclonal Antibody (OTI5G7), TrueMAB
Applications and Tested Dilution
- Western Blot (WB): 1:200–1:1,000
- Immunohistochemistry (Paraffin) (IHC(P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5G7 |
| Immunogen | Human recombinant protein fragment (aa 240–390 of human KRT19 produced in E. coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin 19 belongs to the intermediate filament protein family, showing biochemical diversity among human epithelial tissues.
- Molecular weight: 40–68 kDa
- Isoelectric point: pH 4.9–7.8
- Expressed in epithelial cells, not in hepatocytes
- Useful for distinguishing epithelial malignancies and identifying liver metastasis
Cytokeratin antibodies assist in tumor differential diagnosis via immunohistochemistry and are valuable tools in cytopathology and flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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