Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112)
Kir2.1 (KCNJ2) 단백질 검출용 Mouse Monoclonal Antibody (Clone S112). Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 등 반응성. 고순도 Protein G 정제, 안정적 PBS/glycerol buffer 보관.
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Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Antibody Microarray (AM) | Assay-dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rhesus monkey, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S112 |
| Immunogen | Fusion protein amino acids 41–64 and 189–428 of mouse Kir2.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735239 |
Available Conjugated Formats
Product Specific Information
- 1 µg/mL of MA5-27681 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 45 kDa.
- No cross-reactivity against Kir2.2 or Kir2.3.
Target Information
The KIR2 subunit family includes Kir2.1, Kir2.2, Kir2.3, and Kir2.4.
Unlike G protein-coupled KIR3 subunits, Kir2.1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity.
Kir2.1 is expressed in the superior and inferior colliculi and the pontine region of the brain, moderating synaptic transmission.
In the placenta, Kir2.1 is expressed throughout gestation in cytotrophoblast cells.
In the kidney, Kir2.1 colocalizes with Kir5.1 in the proximal tubule.
Kir2.1, Kir2.2, and Kir2.3 associate with the membrane-associated guanylate kinase synapse-associated protein 97 in the cerebellum and heart.
Phosphorylation of Kir2.2 by protein kinase A inhibits the association with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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