Thermo Fisher Scientific ULK1 Polyclonal Antibody
ULK1 단백질을 인식하는 Sheep 유래 Polyclonal Antibody로, Western blot 및 IHC에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 자가포식 관련 연구에 유용합니다. 동결건조 형태로 제공되며, 4°C 또는 -20°C에서 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 350–410 of human ULK1 conjugated to a blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in rat and mouse.
- Specificity: ULK1.
Target Information
ULK1 is a serine/threonine protein kinase that plays a critical role in the initial stages of autophagy, a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls regulatory function and localization. Knockdown of ULK1 inhibits autophagic response and rapamycin-induced autophagy, indicating a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for proper localization and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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