Thermo Fisher Scientific ULK1 Polyclonal Antibody
ULK1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, Western blot과 IHC에 사용 가능. 인간, 생쥐, 랫트 반응성. 자가포식 관련 연구에 적합하며, 안정적 장기 보관 가능.
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Thermo Fisher Scientific ULK1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:300 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human ULK1 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water. Centrifuge to remove insoluble material.
- The peptide is homologous in rat and mouse.
- Specificity: ULK1.
Target Information
ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital response to nutrient starvation.
The conserved C-terminal domain (CTD) of ULK1 controls regulatory function and localization. Knockdown of ULK1 inhibits autophagy and rapamycin-induced autophagy, suggesting its role downstream of mTOR.
ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are required for correct localization of ULK1 to the pre-autophagosome and for ULK1 protein stability.
ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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