
Thermo Fisher Scientific Skeletal Muscle Actin Polyclonal Antibody
Rabbit 폴리클로날 항체로 인간, 마우스, 랫트의 Skeletal Muscle Actin 검출에 적합. Western blot, IHC, Flow Cytometry 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Skeletal Muscle Actin. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.12 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735448 |
Product Specific Information
- Positive Control: Raji, NIH-3T3, JC, BCL-1, C2C12
- Predicted Reactivity: Rat (100%), Japanese Medaka (98%), Xenopus laevis (98%), Pig (100%), Chicken (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved and play a key role in cell motility, structure, and integrity.
Alpha, beta, and gamma actin isoforms have been identified:
- Alpha actins are a major constituent of the contractile apparatus.
- Beta and gamma actins are involved in the regulation of cell motility.
This actin is an alpha actin found in skeletal muscle. Mutations in this gene are associated with several congenital myopathies, including:
- Nemaline myopathy type 3
- Congenital myopathy with excess thin myofilaments
- Congenital myopathy with cores
- Congenital myopathy with fiber-type disproportion
These conditions lead to muscle fiber defects.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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