Thermo Fisher Scientific Nectin 3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 5 µg/mL

Product Specifications

Product Specific Information

Nectin 3 / CD113 Polyclonal Antibody detects endogenous levels of Nectin 3 / CD113 protein.

Target Information

Nectin-3 is an 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin superfamily.
Nectins are calcium-independent immunoglobulin-like cell-cell adhesion molecules consisting of four members: nectin 1–4.
They interact homophilically and heterophilically to form various cell-cell junctions, including cadherin-based adherens junctions, synaptic junctions, and Sertoli cell-spermatid junctions.

Nectin-2 and Nectin-3 are ubiquitously expressed, while Nectin-1 is abundant in the brain. Nectin-3 exists in three isoforms (alpha, beta, gamma), with Nectin-3 alpha being the largest.
It is predominantly expressed in testis and placental tissues and interacts with afadin, an actin-binding protein, at cadherin-based junctions.
The ectodomains of Nectin-3 and CD155 (Poliovirus Receptor) show strong affinity to each other.
Injection of antibody 103-A1 into seminiferous tubules disrupts actin filaments in Sertoli cells and causes exfoliation of maturing spermatids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.