Thermo Fisher Scientific CD81 Monoclonal Antibody (M38)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 51 publications 51 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Dot blot (DB)

-

View 5 publications 5 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M38

Immunogen

MOLT-4 (human T-ALL cell line) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD81,uniProtId:P60033-1,ncbiNodeId:9606,antigenRange:1-236,antigenLength:236,antigenImageFileName:10630D_CD81_P60033-1_House_mouse.svg,antigenImageFileNamePDP:10630D_CD81_P60033-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_2532984

Product Specific Information

The Exosome - anti-CD81 antibody recognizes human CD81, a member of the tetraspanin family, and is predicted to react with feline and rabbit. The antibody has been verified for western blotting of cellular and exosomal CD81 antigen, with a molecular weight of ~25 kDa, and used at 2 µg/mL under non-reducing conditions. Other applications may work but have not been tested.

Target Information

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.