Thermo Fisher Scientific CD34 Monoclonal Antibody (581), APC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL for 1x10^6 cells

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

581

Immunogen

581 (Class III)

Conjugate

APC APC APC

View additional formats

• Biotin
• FITC
• PE
• PE-Cyanine5.5
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS with sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536501

Product Specific Information

According to the literature this antibody recognizes the CD34 antigen. Each lot of this antibody is tested by flow cytometry using the KG-1a cell line and provides a minimum of 100 tests. For APC conjugates: excitation 600-650 nm/peak emission 660 nm. Matching isotype control is available: Ms IgG1 APC Cat. No. MG105.

For fluorochrome-conjugated antibodies only, light exposure should be avoided. Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.