Thermo Fisher Scientific RNF2 Polyclonal Antibody
RNF2 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체. Western blot에 최적화되어 있으며, 인간 단백질에 반응하고 소, 개, 마우스에서도 예측 반응. 액상 형태로 제공되며 TBS 버퍼와 BSA 포함. -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 0.3–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (PMELYYAPTKEHK) corresponding to the C-terminus amino acids of RNF2 (aa 324–336) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10977801 |
Product Specific Information
- This antibody is predicted to react with bovine, canine, and mouse based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 4,000.
Target Information
RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). It plays a central role in histone code and gene regulation, with activity enhanced by BMI1/PCGF4.
H2AK119Ub serves as a marker for epigenetic transcriptional repression and participates in X chromosome inactivation in female mammals. RNF2 may be involved in both imprinted and random X inactivation.
It is an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PcG PRC1 complex acts via chromatin remodeling and histone modification, maintaining heritable changes in chromatin expressibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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