Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_0062425) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMPK alpha-1,2,uniProtId:Q13131-1,ncbiNodeId:9606,antigenRange:181-185,antigenLength:559,antigenImageFileName:PA5-117258_AMPK_alpha-1,2_Q13131-1_Rabbit.svg,antigenImageFileNamePDP:PA5-117258_AMPK_alpha-1,2_Q13131-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AMPK alpha-1,2,uniProtId:P54646-1,ncbiNodeId:9606,antigenRange:170-174,antigenLength:552,antigenImageFileName:PA5-117258_AMPK_alpha-1,2_P54646-1_Rabbit.svg,antigenImageFileNamePDP:PA5-117258_AMPK_alpha-1,2_P54646-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901888

Product Specific Information

Positive Samples: 293T, C2C12, C6

Immunogen sequence: LRTSC

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.