Thermo Fisher Scientific Phospho-K-Ras (Ser89) Polyclonal Antibody
Phospho-K-Ras (Ser89) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응. Western blot, IHC, ELISA에 사용 가능. 고순도 친화 크로마토그래피 정제, 액상 형태로 안정적 저장. 연구용으로 세포 증식 및 암 관련 Ras 신호 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S89 of human KRAS (NP_0013567151) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.26 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901887 |
Product Specific Information
- Positive Samples: Jurkat, NIH/3T3
- Immunogen sequence: TKSFE
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in regulating cell proliferation and promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner.
Mutations in this gene can result in:
- Leukemia (acute myelogenous, juvenile myelomonocytic)
- Noonan Syndrome
- Gastric cancer
- Cardiofaciocutaneous syndrome 2
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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