Thermo Fisher Scientific CD56 (NCAM) Monoclonal Antibody (TULY56), Super Bright 645, eBioscience

Thermo Fisher Scientific CD56 (NCAM) Monoclonal Antibody (TULY56), Super Bright 645, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 7 publications

Product Specifications

Available Formats

• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395 / 563 / 661 / 737 / 805
• Brilliant Violet 421 / 605 / 650 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 660-120S / 800
• NovaFluor Red 700 / 710 / 725
• NovaFluor Yellow 690 / 810
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702 / 780
• Request custom conjugation available

Product Specific Information

This TULY56 monoclonal antibody reacts with human CD56 (Neural Cell Adhesion Molecule, NCAM).
CD56 is a glycosylated transmembrane molecule expressed by neurons and involved in homotypic neural cell adhesion. In hematopoietic cells, CD56 is expressed on NK cells and NKT subsets.

• TULY56 does not block CMSSB binding, indicating recognition of different epitopes.
• Performs better after fixation/permeabilization than CMSSB.
• Cross-reacts with Rhesus macaque.
• Reported for use in flow cytometric analysis.
• Tested by flow cytometry on normal human peripheral blood cells.
• Use at 5 µL (0.25 µg) per test (100 µL final volume).
• Recommended cell number: 10⁵–10⁸ cells/test.

Fluorochrome Characteristics:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Use a 660/20 bandpass filter. Protect from light due to photo-oxidation sensitivity.

Staining Panel Recommendation:
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.

Fixation Compatibility:
Samples can be fixed in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C with minimal brightness loss.

Laser Information:
Excitation: 405 nm
Emission: 645 nm
Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD56 (NCAM) is a transmembrane glycoprotein of the immunoglobulin family expressed in the nervous system (120–180 kDa isoforms).
It mediates cell adhesion, migration, axonal growth, and synaptic plasticity. Polysialic modification reduces adhesion.
CD56 interacts with extracellular matrix components such as laminin and integrins, and is expressed in neuroectodermal tissues and tumors (retinoblastoma, medulloblastoma, astrocytoma, neuroblastoma).
It is a sensitive neuroendocrine marker for neuroendocrine and ovarian granulosa cell tumors.
Associated diseases include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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